• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PCNP Recombinant monoclonal antibody (86075-1-RR)

    WB analysis using 86075-1-RR (same clone as 86075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86075-1-RR (PCNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PCNP Recombinant monoclonal antibody (86075-1-RR)

    IHC staining of human colon using 86075-1-RR (same clone as 86075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86075-1-RR (PCNP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PCNP Recombinant monoclonal antibody (86075-1-RR)

    IHC staining of human colon using 86075-1-RR (same clone as 86075-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86075-1-RR (PCNP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCNP Recombinant monoclonal antibody (86075-1-RR)

    IF Staining of HeLa using 86075-1-RR (same clone as 86075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (86075-1-RR, Clone: 250576B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86075-1-RR

    Affinity and Kinetic Characterization of 86075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86075-1-RR against Human PCNP were performed. The affinity constant is 1.41 nM.

PCNP Recombinant monoclonal antibody, PBS Only

PCNP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86075-1-PBS
Clone No.250576B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86075-1-PBS targets PCNP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag30951

Product name: Recombinant human PCNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of BC013916

Sequence: MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKI

相同性解析による交差性が予測される生物種
Full Name PEST proteolytic signal containing nuclear protein
Calculated molecular weight 19 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_3744602
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WW12
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PEST-containing nuclear protein (PCNP), characterized by a PEST motif rich in proline (P), glutamic acid (E), serine (S), and threonine (T), is firstly identified by database mining. The PCNP protein with the PEST motif is highly subjected to ubiquitination. PCNP may act as a transcriptional factor, cell cycle regulatory protein and tumor regulating nuclear protein.(PMID: 35813472, PMID: 32548978, PMID: 31487123)

||
New chat

Able™

正在加载，请稍候...