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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCNP Recombinant antibody (86075-1-RR)

    WB analysis using 86075-1-RR (same clone as 86075-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86075-1-RR (PCNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCNP Recombinant antibody (86075-1-RR)

    IF Staining of HeLa using 86075-1-RR (same clone as 86075-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (86075-1-RR, Clone: 250576B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86075-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86075-1-RR

    Affinity and Kinetic Characterization of 86075-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86075-1-RR against Human PCNP were performed. The affinity constant is 1.41 nM.

PCNP Recombinant monoclonal antibody, PBS Only

PCNP Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86075-1-PBS
Clone No.250576B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86075-1-PBS targets PCNP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag30951

Product name: Recombinant human PCNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of BC013916

Sequence: MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKI

相同性解析による交差性が予測される生物種
Full Name PEST proteolytic signal containing nuclear protein
Calculated molecular weight 19 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WW12
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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