• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCNP Polyclonal antibody

PCNP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29305-1-AP

Synonyms

DKFZp781I24156, PCNP

Validation Data Gallery

Applications:
  • WB analysis using 29305-1-AP

    WB analysis using 29305-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29305-1-AP (PCNP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29305-1-AP

    IHC staining of human prostate cancer using 29305-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29305-1-AP (PCNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA2780 cells, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29305-1-AP targets PCNP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNP fusion protein Ag30951 相同性解析による交差性が予測される生物種
Full Name PEST proteolytic signal containing nuclear protein
Calculated molecular weight 19 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC013916
Gene symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2918277
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCNP antibody 29305-1-APDownload protocol
IHC protocol for PCNP antibody 29305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols