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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCNP Polyclonal antibody (11180-2-AP)

    WB analysis using 11180-2-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11180-2-AP (PCNP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PCNP Polyclonal antibody (11180-2-AP)

    WB analysis of HepG2 using 11180-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11180-2-AP (PCNP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PCNP Polyclonal antibody (11180-2-AP)

    IP experiment of HepG2 using 11180-2-AP

    IP result of anti-PCNP (IP:11180-2-AP, 4ug; Detection:11180-2-AP 1:600) with HepG2 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PCNP Polyclonal antibody (11180-2-AP)

    IHC staining of human lymphoma using 11180-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11180-2-AP (PCNP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PCNP Polyclonal antibody (11180-2-AP)

    IHC staining of human lymphoma using 11180-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11180-2-AP (PCNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCNP Polyclonal antibody (11180-2-AP)

    IF Staining of HeLa using 11180-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCNP antibody (11180-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

PCNP Polyclonal antibody

PCNP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11180-2-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PEST-containing nuclear protein, PEST proteolytic signal-containing nuclear protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11180-2-AP targets PCNP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1663

Product name: Recombinant human PCNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-130 aa of BC013916

Sequence: MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAADLPTKPTKISKFGFAIGSQTTKKASAISIKLGSSKPKETVPTLAPKTLSVAAAFNEDEDGYTNISWTKLLQ

相同性解析による交差性が予測される生物種
Full Name PEST proteolytic signal containing nuclear protein
Calculated molecular weight 19 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2160652
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCNP can be detected as 24-28 kDa after posttranslational modification.

Protocols

Product Specific Protocols
IF protocol for PCNP antibody 11180-2-APDownload protocol
IHC protocol for PCNP antibody 11180-2-APDownload protocol
IP protocol for PCNP antibody 11180-2-APDownload protocol
WB protocol for PCNP antibody 11180-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition.

Authors - Pengzhen Dong
View Article
humanWB,IHC,IF

BMC Cancer

PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.

Authors - Dong-Dong Wu
  • KD Validated
View Article
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