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PCNP Polyclonal antibody

PCNP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11180-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

PEST-containing nuclear protein, PEST proteolytic signal-containing nuclear protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11180-2-AP targets PCNP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNP fusion protein Ag1663 相同性解析による交差性が予測される生物種
Full Name PEST proteolytic signal containing nuclear protein
Calculated molecular weight 19 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2160652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCNP can be detected as 24-28 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for PCNP antibody 11180-2-APDownload protocol
IHC protocol for PCNP antibody 11180-2-APDownload protocol
IF protocol for PCNP antibody 11180-2-APDownload protocol
IP protocol for PCNP antibody 11180-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition.

Authors - Pengzhen Dong
humanWB,IHC,IF

BMC Cancer

PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.

Authors - Dong-Dong Wu
  • KD Validated
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