Validation Data Gallery
Tested Applications
| Positive WB detected in | 293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65034-1-Ig targets PCNA in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | proliferating cell nuclear antigen |
| Calculated molecular weight | 29 kDa/31 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC000491 |
| Gene Symbol | PCNA |
| Gene ID (NCBI) | 5111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. |
Background Information
PCNA, also named as Cyclin, belongs to the PCNA family. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. The PCNA protein has been highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. This antibody is a mouse monoclonal antibody raised against recombinant protein of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa （PMID: 1358458）.