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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCK2 Recombinant monoclonal antibody (88027-1-RR)

    WB analysis using 88027-1-RR (same clone as 88027-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88027-1-RR (PCK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88027-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCK2 Recombinant monoclonal antibody (88027-1-RR)

    IF Staining of HepG2 using 88027-1-RR (same clone as 88027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCK2 antibody (88027-1-RR, Clone: 260096E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88027-1-PBS in a different storage buffer formulation.

PCK2 Recombinant monoclonal antibody, PBS Only

PCK2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 88027-1-PBS
Clone No.260096E12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M, Phosphoenolpyruvate carboxykinase 2, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88027-1-PBS targets PCK2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6681

Product name: Recombinant human PCK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-640 aa of BC001454

Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Calculated molecular weight 71 kDa
Observed molecular weight 66-71 kDa
GenBank accession numberBC001454
Gene Symbol PCK2
Gene ID (NCBI) 5106
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16822
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody specifically recognizes PCK2.

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