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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PCK2 Polyclonal antibody (14892-1-AP)

    WB analysis of mouse brain using 14892-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PCK2 Polyclonal antibody (14892-1-AP)

    WB analysis of rat brain using 14892-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PCK1/2 Polyclonal antibody (14892-1-AP)

    WB analysis of mouse liver using 14892-1-AP (same clone as 14892-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PCK2 Polyclonal antibody (14892-1-AP)

    IHC staining of human breast cancer using 14892-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PCK2 Polyclonal antibody (14892-1-AP)

    IHC staining of human breast cancer using 14892-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PCK1/2 Polyclonal antibody (14892-1-AP)

    IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PCK1/2 Polyclonal antibody (14892-1-AP)

    IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCK1/2 Polyclonal antibody (14892-1-AP)

    IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCK1/2 Polyclonal antibody (14892-1-AP)

    IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCK1/2 Polyclonal antibody (14892-1-AP)

    IF Staining of MCF-7 using 14892-1-AP (same clone as 14892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK1/2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PCK1/2 Polyclonal antibody (14892-1-AP)

    FC experiment of HepG2 using 14892-1-AP (same clone as 14892-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PCK1/2 Polyclonal antibody (14892-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

PCK1/2 Polyclonal antibody, PBS Only

PCK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 14892-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

PCK2, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14892-1-PBS targets PCK1/2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6681

Product name: Recombinant human PCK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-640 aa of BC001454

Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Calculated molecular weight 71 kDa
Observed molecular weight 66-71 kDa
GenBank accession numberBC001454
Gene Symbol PCK2
Gene ID (NCBI) 5106
RRIDAB_2160044
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16822
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.

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