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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PCF11 Polyclonal antibody (23540-1-AP)

    WB analysis of K-562 using 23540-1-AP (same clone as 23540-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PCF11 Polyclonal antibody (23540-1-AP)

    WB analysis of HeLa using 23540-1-AP (same clone as 23540-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PCF11 Polyclonal antibody (23540-1-AP)

    IP experiment of Jurkat using 23540-1-AP (same clone as 23540-1-PBS)

    IP result of anti-PCF11 (IP:23540-1-AP, 4ug; Detection:23540-1-AP 1:2000) with Jurkat cells lysate 1400 ug. This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PCF11 Polyclonal antibody (23540-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 23540-1-AP (same clone as 23540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 23540-1-AP (PCF11 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PCF11 Polyclonal antibody, PBS Only

PCF11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 23540-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

KIAA0824, Pre-mRNA cleavage complex 2 protein Pcf11, Pre-mRNA cleavage complex II protein Pcf11

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23540-1-PBS targets PCF11 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20263

Product name: Recombinant human PCF11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1206-1555 aa of BC146778

Sequence: IPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

相同性解析による交差性が予測される生物種
Full Name PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
Calculated molecular weight 1555 aa, 173 kDa
Observed molecular weight 173 kDa
GenBank accession numberBC146778
Gene Symbol PCF11
Gene ID (NCBI) 51585
RRIDAB_2879293
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO94913
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. This antibody is specific to the 173kd human PCF11 protein.

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