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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PCDHGC5 Polyclonal antibody (23848-1-AP)

    IHC staining of human lung cancer using 23848-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23848-1-AP (PCDHGC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PCDHGC5 Polyclonal antibody (23848-1-AP)

    IHC staining of human lung cancer using 23848-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23848-1-AP (PCDHGC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PCDHGC5 Polyclonal antibody

PCDHGC5 Polyclonal Antibody for IHC, ELISA
Cat No. 23848-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Protocadherin gamma-C5, PCDHGC, PCDH-gamma-C5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23848-1-AP targets PCDHGC5 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHGC5 fusion protein Ag20867 相同性解析による交差性が予測される生物種
Full Name protocadherin gamma subfamily C, 5
Calculated molecular weight581aa,65 kDa; 944aa,102 kDa
GenBank accession numberBC112081
Gene Symbol PCDHGC5
Gene ID (NCBI) 56097
RRIDAB_3669420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y5F6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). Disruption of the γ-protocadherins locus causes neonatal lethality, and Pcdhgc3, Pcdhgc4, and Pcdhgc5 are essential for the observed lethality.

Protocols

Product Specific Protocols
IHC protocol for PCDHGC5 antibody 23848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols