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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PCDHB5 Polyclonal antibody (15245-1-AP)

    WB analysis of K-562 using 15245-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15245-1-AP (PCDHB5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PCDHB5 Polyclonal antibody (15245-1-AP)

    WB analysis of HepG2 using 15245-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15245-1-AP (PCDHB5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

PCDHB5 Polyclonal antibody

PCDHB5 Polyclonal Antibody for WB, ELISA
Cat No. 15245-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PCDH beta 5, PCDH BETA5, PCDHB5, protocadherin beta 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15245-1-AP targets PCDHB5 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7458

Product name: Recombinant human PCDHB5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 382-690 aa of BC001186

Sequence: CSIQNDLPFLLKPTLKNFYTLVTQRTLDRESQAEYNITITVTDMGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQNPHLRLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFSMWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLV

相同性解析による交差性が予測される生物種
Full Name protocadherin beta 5
Calculated molecular weight 86 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC001186
Gene Symbol PCDHB5
Gene ID (NCBI) 26167
RRIDAB_2159139
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5E4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCDHB5 antibody 15245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols