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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHA5 Polyclonal antibody (15270-1-AP)

    WB analysis using 15270-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    WB analysis of human brain using 15270-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human brain using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human brain using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human gliomas using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human gliomas using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of rat cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of rat cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of mouse cerebellum using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human lung using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human lung using 15270-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens).

PCDHA5 Polyclonal antibody

PCDHA5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15270-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PCDH ALPHA5, PCDH alpha 5, CRNR6, CNRS6, CNRN6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inunboiled mouse brain tissue, human brain tissue, unboiled rat brain tissue
Positive IHC detected inmouse cerebellum tissue, human brain tissue, human gliomas tissue, human lung tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15270-1-AP targets PCDHA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7376

Product name: Recombinant human PCDHA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-400 aa of BC033735

Sequence: QLHYSIPEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGRGDLLEVNLQNGILFVNSRIDREELCRRRAECSIHLEVIVDRPLQVFHVEVAVKDINDNPPRFSRQEQRLFILESRMPDSRFPLEGASDLDIGANAQLRYRLNPNEYFDLDVKTNEEETNFLELVLRKSLDREETQEHRLLVIATDGGKPELTGTVQLLINVLDANDSAPEFDKSIYNVRLLENAPSGTLVIKLNASDADEGINKEIVYFFSNLVLDDVKSKFIINSNTGEIKVNGELDYEDYNSYEINIDAMDKSTFPLSGHCKVVVKLLDVNDNTPEMAITTLFLPVKEDAPLSTVIALISVSDRDSGANGQVTCSLMPHVPFKLVSTF

相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 5
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC033735
Gene Symbol PCDHA5
Gene ID (NCBI) 56143
RRIDAB_2283415
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCDHA5 antibody 15270-1-APDownload protocol
IHC protocol for PCDHA5 antibody 15270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols