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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHA3 Polyclonal antibody (18803-1-AP)

    WB analysis using 18803-1-AP (same clone as 18803-1-PBS)

    Calu-3 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCDHA3 Polyclonal antibody (18803-1-AP)

    WB analysis using 18803-1-AP (same clone as 18803-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    WB analysis of mouse colon using 18803-1-AP (same clone as 18803-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of mouse brain using 18803-1-AP (same clone as 18803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of mouse brain using 18803-1-AP (same clone as 18803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of human brain using 18803-1-AP (same clone as 18803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of human brain using 18803-1-AP (same clone as 18803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18803-1-PBS in a different storage buffer formulation.

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PCDHA3 Polyclonal antibody, PBS Only

PCDHA3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18803-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Protocadherin alpha-3, PCDH-alpha-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18803-1-PBS targets PCDHA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 3
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC129807
Gene Symbol PCDHA3
Gene ID (NCBI) 56145
RRIDAB_10596786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5H8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCDHA3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. The antibody can recognize both the isoforms of PCDHA3.