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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis using 10127-2-AP (same clone as 10127-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of Raji using 10127-2-AP (same clone as 10127-2-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of human heart using 10127-2-AP (same clone as 10127-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of human brain using 10127-2-AP (same clone as 10127-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PCDHA2 Polyclonal antibody (10127-2-AP)

    IP experiment of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

    IP result of anti-PCDHA2 (IP:10127-2-AP, 3ug; Detection:10127-2-AP 1:300) with HeLa cells lysate 4650ug. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

PCDHA2 Polyclonal antibody, PBS Only

PCDHA2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 10127-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10127-2-PBS targets PCDHA2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0173

Product name: Recombinant human PCDHA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 98-259 aa of BC003126

Sequence: RSAECSIHVEVIVDRPLQVFHVEVEVKDINDNPPIFPMTVKTIRFPESRLLDSRFPLEGASDADIGVNALLSYKLSSSEFFFLDIQANDELSESLSLVLGKSLDREETAEVNLLLVATDGGKPELTGTVQILIKVLDVNDNEPTFAQSVYKVKLLENTANGT

相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa, 80 kDa
GenBank accession numberBC003126
Gene Symbol PCDHA2
Gene ID (NCBI) 56146
RRIDAB_2158964
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5H9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).

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