Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells, U2OS cells, human brain tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31671-1-AP targets PCDHA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36313 Product name: Recombinant human PCDHA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 130-310 aa of BC166697 Sequence: PPVFRGREQIIFIPESRLLNSRFPIEGAADADIGANALLTYTLSPSDYFSLDVEASDELSKSLWLELRKYLDREETPELHLLLTATDGGKPELQGTVELLITVLDVNDNAPLFDQAVYRVHLLETTANGTLVTTLNASDADEGVNGEVVFSFDSGISRDIQEKFKVDSSSGEIRLIDKLDY 相同性解析による交差性が予測される生物種 |
| Full Name | protocadherin alpha 1 |
| Observed molecular weight | 102 kDa |
| GenBank accession number | BC166697 |
| Gene Symbol | PCDHA1 |
| Gene ID (NCBI) | 56147 |
| RRID | AB_3670070 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y5I3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protocadherin alpha-1 (PCDHA1) is a potential calcium-dependent cell adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDHA1 antibody 31671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |