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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDH8 Polyclonal antibody (14000-1-AP)

    WB analysis using 14000-1-AP (same clone as 14000-1-PBS)

    mouse brain and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14000-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCDH8 Polyclonal antibody (14000-1-AP)

    WB analysis using 14000-1-AP (same clone as 14000-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14000-1-PBS in a different storage buffer formulation.

PCDH8 Polyclonal antibody, PBS Only

PCDH8 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 14000-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14000-1-PBS targets PCDH8 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5090

Product name: Recombinant human PCDH8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-351 aa of BC036025

Sequence: TVRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFNSSLLRVREGDGQLTVGDAGLDRERLCGQAPQCVLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQIPVEVSEGAAVGTRIPLEVPVDEDVGANGLQTVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQAAYSLELVAQDGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEGPNGDVVFAFGARTPPEARRLFRLDPRSGRLTLAGPVDYERQDTYELDVRAQDRGPGPRAATCKVIVRIRDVNDNA

相同性解析による交差性が予測される生物種
Full Name protocadherin 8
Calculated molecular weight 1070 aa, 113 kDa
Observed molecular weight104 kDa,113 kDa
GenBank accession numberBC036025
Gene Symbol PCDH8
Gene ID (NCBI) 5100
RRIDAB_3669175
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95206
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.

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