Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse retina tissue, rat retina tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17424-1-AP targets PCDH21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCDH21 fusion protein Ag11451 相同性解析による交差性が予測される生物種 |
| Full Name | protocadherin 21 |
| Calculated molecular weight | 745 aa, 82 kDa |
| Observed molecular weight | 110-120 kDa |
| GenBank accession number | BC038799 |
| Gene Symbol | PCDH21 |
| Gene ID (NCBI) | 92211 |
| RRID | AB_3669273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JP9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). PCDH21 (protocadherin 21), also known as CDHR1, is a photoreceptor-specific cadherin protein containing six ECs and an intracellular domain. The mutations of PCDH21 cause recessive retinal degeneration and autosomal recessive cone-rod dystrophy in humans (PMID: 16288196; PMID: 20805371). The full length of the PCDH21 protein is 120 kDa, the N-terminal soluble fragment is 95 kDa, and the C-terminal fragment is 25 kDa (PMID: 35066146; PMID: 18654668; PMID: 33964272).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDH21 antibody 17424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |