Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34123-1-AP targets PCDH18 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41285 Product name: Recombinant human PCDH18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 750-900 aa of BC093815 Sequence: PSRQIHKGDITLVPTINGTLPIRSHHRSSPSSSPTLERGQMGSRQSHNSHQSLNSLVTISSNHVPENFSLELTHATPAVEQVSQLLSMLHQGQYQPRPSFRGNKYSRSYRYALQDMDKFSLKDSGRGDSEAGDSDYDLGRDSPIDRLLGEG 相同性解析による交差性が予測される生物種 |
| Full Name | protocadherin 18 |
| Calculated molecular weight | 1135 aa, 126 kDa |
| GenBank accession number | BC093815 |
| Gene Symbol | PCDH18 |
| Gene ID (NCBI) | 54510 |
| RRID | AB_3743157 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9HCL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protocadherin 18 (PCDH18) is a member of the non-clustered protocadherin subfamily within the cadherin superfamily. Encoded by the PCDH18 gene on chromosome 4q28, it contains six to seven extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. PCDH18 is expressed in brain, heart, kidney, lung, and trachea. It plays critical roles in cell-cell adhesion, neuronal migration, axon guidance, and synapse formation during development. Emerging evidence links PCDH18 mutations or dysregulation to neurodevelopmental disorders, including autism spectrum disorder and intellectual disability, as well as to certain cancers (PMID: 22450339; PMID: 29075151; PMID: 28588296).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCDH18 antibody 34123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |