Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, K-562 cells, MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16141-1-AP targets PCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9202 Product name: Recombinant human PCBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC012061 Sequence: MESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKKMREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLEAYTIQGQYAIPHPDLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGTL 相同性解析による交差性が予測される生物種 |
| Full Name | poly(rC) binding protein 3 |
| Calculated molecular weight | 313 aa, 33 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC012061 |
| Gene Symbol | PCBP3 |
| Gene ID (NCBI) | 54039 |
| RRID | AB_3085481 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCBP3 antibody 16141-1-AP | Download protocol |
| WB protocol for PCBP3 antibody 16141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
