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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCBD1 Polyclonal antibody (15702-1-AP)

    WB analysis using 15702-1-AP (same clone as 15702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15702-1-AP (PCBD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse liver using 15702-1-AP (same clone as 15702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of human liver cancer using 15702-1-AP (same clone as 15702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse kidney using 15702-1-AP (same clone as 15702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse kidney using 15702-1-AP (same clone as 15702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PCBD1 Polyclonal antibody (15702-1-AP)

    IHC staining of mouse liver using 15702-1-AP (same clone as 15702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15702-1-AP (PCBD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCBD1 Polyclonal antibody (15702-1-AP)

    IF Staining of HeLa using 15702-1-AP (same clone as 15702-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCBD1 antibody (15702-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15702-1-PBS in a different storage buffer formulation.

PCBD1 Polyclonal antibody, PBS Only

PCBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15702-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

4-alpha-hydroxy-tetrahydropterin dehydratase, DCOH, Dimerization cofactor of hepatocyte nuclear factor 1-alpha, EC:4.2.1.96, PCBD

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15702-1-PBS targets PCBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8331

Product name: Recombinant human PCBD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-104 aa of BC006324

Sequence: MAGKAHRLSAEERDQLLPNLRAVGWNELEGRDAIFKQFHFKDFNRAFGFMTRVALQAEKLDHHPEWFNVYNKVHITLSTHECAGLSERDINLASFIEQVAVSMT

相同性解析による交差性が予測される生物種
Full Name pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Calculated molecular weight 104 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC006324
Gene Symbol PCBD1
Gene ID (NCBI) 5092
RRIDAB_2267945
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP61457
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCBD1, also known as DCOH and PCBD, belongs to the pterin-4-alpha-carbinolamine dehydratase family. PCBD1 is a bifunctional protein that acts as an enzyme in the regeneration of cofactor tetrahydrobiopterin (BH4), crucial for the function of aromatic amino acid hydroxylases, and as a dimerization cofactor of transcription factors HNF1A and HNF1B, important in liver and pancreas development and function. PCBD1 is expressed in the developing pancreas. The calculated molecular weight of PCBD1 is 12 kDa (PMID: 24204001, 24848070).

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