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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PBX1 Recombinant monoclonal antibody (86504-3-RR)

    WB analysis of HeLa using 86504-3-RR (same clone as 86504-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 86504-3-RR (PBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PBX1 Recombinant monoclonal antibody (86504-3-RR)

    WB analysis using 86504-3-RR (same clone as 86504-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86504-3-RR (PBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PBX1 Recombinant monoclonal antibody (86504-3-RR)

    IHC staining of human stomach using 86504-3-RR (same clone as 86504-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 86504-3-RR (PBX1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PBX1 Recombinant monoclonal antibody (86504-3-RR)

    IF Staining of HeLa using 86504-3-RR (same clone as 86504-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX1 antibody (86504-3-RR, Clone: 251373B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86504-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86504-3-RR

    Affinity and Kinetic Characterization of 86504-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86504-3-RR against Human PBX1 were performed. The affinity constant is 0.342 nM.

PBX1 Recombinant monoclonal antibody, PBS Only

PBX1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86504-3-PBS
Clone No.251373B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Homeobox protein PBX1, Homeobox protein PRL, pre B cell leukemia homeobox 1, Pre-B-cell leukemia transcription factor 1, PRL

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86504-3-PBS targets PBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12817

Product name: Recombinant human PBX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC101578

Sequence: MDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAGSDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANIYAAKTAVTATNVSAHGSQANSPSTPNSAGSSSSFNMSNSGDLFMS

相同性解析による交差性が予測される生物種
Full Name pre-B-cell leukemia homeobox 1
Calculated molecular weight 430 aa, 47 kDa
Observed molecular weight 38 kDa, 47 kDa
GenBank accession numberBC101578
Gene Symbol PBX1
Gene ID (NCBI) 5087
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP40424
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b: MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b), and molecular weight of PBX1 is 47 kDa and 38 kDa.

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