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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Recombinant monoclonal antibody (81032-1-RR)

    IHC staining of human ovary tumor using 81032-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81032-1-RR (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PAX8 Recombinant monoclonal antibody (81032-1-RR)

    IHC staining of human thyroid cancer using 81032-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81032-1-RR (PAX8 antibody) at dilution of 1:2800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PAX8 Recombinant monoclonal antibody (81032-1-RR)

    IHC staining of human thyroid cancer using 81032-1-RR

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81032-1-RR (PAX8 antibody) at dilution of 1:2800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Recombinant monoclonal antibody (81032-1-RR)

    IHC staining of human ovary tumor using 81032-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81032-1-RR (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PAX8 Recombinant monoclonal antibody (81032-1-RR)

    IF Staining of SKOV-3 using 81032-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (81032-1-RR, Clone: 6C24 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity of 81032-1-RR

    Affinity of 81032-1-RR

    Biolayer interferometry (BLI) kinetic assays of 81032-1-RR against Human PAX8 were performed. The affinity constant is 1.07 nM.

PAX8 Recombinant monoclonal antibody

PAX8 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 81032-1-RR
Clone No.6C24

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

PAX-8, paired box 8, Paired box protein Pax 8, Paired box protein Pax-8, PAX 8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman ovary tumor tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81032-1-RR targets PAX8 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0306

Product name: Recombinant human PAX8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-212 aa of BC001060

Sequence: MPHNSIRSGHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIRTKVQQPFNLPMDSCVATKSLSPGHTLIPSSAVTPPESPQSDSLGSTYSINGLLGIAQPGSDKRKMDDSDQDSCRLSIDS

相同性解析による交差性が予測される生物種
Full Name paired box 8
Calculated molecular weight 48 kDa
GenBank accession numberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_3743364
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.

Protocols

Product Specific Protocols
IF protocol for PAX8 antibody 81032-1-RRDownload protocol
IHC protocol for PAX8 antibody 81032-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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