• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using PASK Polyclonal antibody (14396-1-AP)

    WB analysis of HEK-293 using 14396-1-AP (same clone as 14396-1-PBS)

    WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PASK Polyclonal antibody (14396-1-AP)

    WB analysis using 14396-1-AP (same clone as 14396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PASK Polyclonal antibody (14396-1-AP)

    WB analysis of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using PASK Polyclonal antibody (14396-1-AP)

    IP experiment of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

    IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human prostate hyperplasia using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human prostate hyperplasia using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human testis using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human testis using 14396-1-AP (same clone as 14396-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PASK Polyclonal antibody (14396-1-AP)

    IF Staining of HeLa using 14396-1-AP (same clone as 14396-1-PBS)

    Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PASK Polyclonal antibody, PBS Only

PASK Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14396-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14396-1-PBS targets PASK in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5893

Product name: Recombinant human PASK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 999-1323 aa of BC063585

Sequence: YSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS

相同性解析による交差性が予測される生物種
Full Name PAS domain containing serine/threonine kinase
Calculated molecular weight 143 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC063585
Gene Symbol PASK
Gene ID (NCBI) 23178
RRIDAB_2878053
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RG2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.

||
New chat

Able™

正在加载，请稍候...