Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse spleen tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
14463-1-AP targets PARVB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5760 Product name: Recombinant human PARVB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-364 aa of BC046103 Sequence: MSSAPRSPTPRPRRMKKDESFLGKLGGTLARKRRAREVSDLQEEGKNAINSPMSPALADVHPEDTQLEENEERTMIDPTSKEDPKFKELVKVLLDWINDVLVEERIIVKQLEEDLYDGQVLQKLLEKLAGCKLNVAEVTQSEIGQKQKLQTVLEAVHDLLRPRGWALRWSVDSIHGKNLVAILHLLVSLAMHFRAPIRLPEHVTVQVVVVRKREGLLHSSHISEELTTTTEMMMGRFERDAFDTLFDHAPDKLSVVKKSLITFVNKHLNKLNLEVTELETQFADGVYLVLLMGLLEDYFVPLHHFYLTPESFDQKVHNVSFAFELMLDGGLKKPKARPEDVVNLDLKSTLRVLYNLFTKYKNVE 相同性解析による交差性が予測される生物種 |
| Full Name | parvin, beta |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42 kDa and 45 kDa |
| GenBank accession number | BC046103 |
| Gene Symbol | PARVB |
| Gene ID (NCBI) | 29780 |
| RRID | AB_2158952 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARVB antibody 14463-1-AP | Download protocol |
| IHC protocol for PARVB antibody 14463-1-AP | Download protocol |
| IF protocol for PARVB antibody 14463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase Inhibitors. | ||
Oncogene A critical role of integrin-linked kinase, ch-TOG and TACC3 in centrosome clustering in cancer cells.
| ||
J Biol Chem Disruption of the integrin-linked kinase (ILK) pseudokinase domain affects kidney development in mice. | ||
J Cell Physiol The Integrin-Mediated ILK-Parvin-αPix Signalling Axis Controls Differentiation in Mammary Epithelial Cells. | ||
Sci Rep Integrin-Linked Kinase links Dynactin-1/Dynactin-2 with cortical Integrin receptors to orient the mitotic spindle relative to the substratum.
|