Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of U2OS using 55268-1-AP
U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55268-1-AP
WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.
WB analysis of mouse kidney using 55268-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of U2OS using 55268-1-AP
IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.
IHC staining of human stomach cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 55268-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MDCK using 55268-1-AP
Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Alpha Parvin/Actopaxin Polyclonal antibody

Alpha Parvin/Actopaxin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55268-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Alpha parvin, Actopaxin, PARVA, α-Parvin, CH-ILKBP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	U2OS cells, HeLa cells, mouse kidney tissue
Positive IP detected in	U2OS cells
Positive IHC detected in	human stomach cancer tissue, human heart tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
CoIP	See 1 publications below

Product Information

55268-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	parvin, alpha
Calculated molecular weight	42 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	NM_018222
Gene Symbol	Alpha Parvin/PARVA
Gene ID (NCBI)	55742
RRID	AB_10951112
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NVD7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

Protocols

Product Specific Protocols
IF protocol for Alpha Parvin/Actopaxin antibody 55268-1-AP	Download protocol
IHC protocol for Alpha Parvin/Actopaxin antibody 55268-1-AP	Download protocol
IP protocol for Alpha Parvin/Actopaxin antibody 55268-1-AP	Download protocol
WB protocol for Alpha Parvin/Actopaxin antibody 55268-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,CoIP	Proteome Sci Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium. Authors - Lancia N F Darville View Article
human	WB	Nat Commun Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis Authors - Yesheng Fu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of U2OS using 55268-1-AP

WB analysis of HeLa using 55268-1-AP

WB analysis of mouse kidney using 55268-1-AP

IP experiment of U2OS using 55268-1-AP

IHC staining of human stomach cancer using 55268-1-AP

IHC staining of human liver cancer using 55268-1-AP