Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 17535-1-AP (same clone as 17535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17535-1-AP (PARP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IP experiment of MCF-7 using 17535-1-AP (same clone as 17535-1-PBS)
IP result of anti-PARP9 (IP:17535-1-AP, 4ug; Detection:17535-1-AP 1:3000) with MCF-7 cells lysate 1400 ug. This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17535-1-AP (PARP9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17535-1-AP (PARP9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human ovary using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 17535-1-AP (same clone as 17535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 17535-1-AP (PARP9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.
IF Staining of THP-1 using 17535-1-AP (same clone as 17535-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PARP9 antibody (17535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17535-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PARP9 Polyclonal antibody, PBS Only

PARP9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 17535-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 9, ARTD9, BAL, BAL1, EC:2.4.2.-

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17535-1-PBS targets PARP9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11587 Product name: Recombinant human PARP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 476-819 aa of BC039580 Sequence: EARSPAINLMGFNVEEMYEAHAWIQRILSLQNHHIIENNHILYLGRKEHDILSQLQKTSSVSITEIISPGRTELEIEGARADLIEVVMNIEDMLCKVQEEMARKKERGLWRSLGQWTIQQQKTQDEMKENIIFLKCPVPPTQELLDQKKQFEKCGLQVLKVEKIDNEVLMAAFQRKKKMMEEKLHRQPVSHRLFQQVPYQFCNVVCRVGFQRMYSTPCDPKYGAGIYFTKNLKNLAEKAKKISAADKLIYVFEAEVLTGFFCQGHPLNIVPPPLSPGAIDGHDSVVDNVSSPETFVIFSGMQAIPQYLWTCTQEYVQSQDYSSGPMRPFAQHPWRGFASGSPVD 相同性解析による交差性が予測される生物種
Full Name	poly (ADP-ribose) polymerase family, member 9
Calculated molecular weight	819 aa, 92 kDa
Observed molecular weight	88 kDa
GenBank accession number	BC039580
Gene Symbol	PARP9
Gene ID (NCBI)	83666
RRID	AB_2158929
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IXQ6
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNγ, inducing the transcription of IFNγ-controlled genes.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17535-1-AP (same clone as 17535-1-PBS)

IP experiment of MCF-7 using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of mouse brain using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human kidney using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human kidney using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human skin using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human skin using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human lung using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human lung using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human ovary using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human ovary using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human lymphoma using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human lymphoma using 17535-1-AP (same clone as 17535-1-PBS)

IHC staining of human heart using 17535-1-AP (same clone as 17535-1-PBS)