Validation Data Gallery
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Tested Applications
| Positive IP detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17754-1-AP targets PARP8 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARP8 fusion protein Ag12111 相同性解析による交差性が予測される生物種 |
| Full Name | poly (ADP-ribose) polymerase family, member 8 |
| Calculated molecular weight | 812 aa, 91 kDa |
| Observed molecular weight | 91-96 kDa |
| GenBank accession number | BC075801 |
| Gene Symbol | PARP8 |
| Gene ID (NCBI) | 79668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3A8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein mono-ADP-ribosyltransferase PARP8, also known as ADP-ribosyltransferase diphtheria toxin-like 16 (ARTD16), is primarily localised on the nuclear envelope for the majority of the cell cycle but localises to centrosomes and spindle poles during mitosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PARP8 antibody 17754-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |