• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PARP4 Polyclonal antibody (19775-1-AP)

    WB analysis using 19775-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PARP4 Polyclonal antibody (19775-1-AP)

    WB analysis of A549 using 19775-1-AP

    WB result of PARP4 antibody (19775-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP4 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using PARP4 Polyclonal antibody (19775-1-AP)

    WB analysis using 19775-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PARP4 Polyclonal antibody (19775-1-AP)

    IHC staining of mouse kidney using 19775-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PARP4 Polyclonal antibody (19775-1-AP)

    IHC staining of human stomach cancer using 19775-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PARP4 Polyclonal antibody (19775-1-AP)

    IF Staining of HepG2 using 19775-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PARP4 antibody (19775-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

PARP4 Polyclonal antibody

PARP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19775-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 4, ADPRTL1, ARTD4, EC:2.4.2.-, p193

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inmouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19775-1-AP targets PARP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 4
Calculated molecular weight 193 kDa
Observed molecular weight193 kDa
GenBank accession numberNM_006437
Gene Symbol PARP4
Gene ID (NCBI) 143
RRIDAB_3085585
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.

Protocols

Product Specific Protocols
IF protocol for PARP4 antibody 19775-1-APDownload protocol
IHC protocol for PARP4 antibody 19775-1-APDownload protocol
WB protocol for PARP4 antibody 19775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...