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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis using 11289-1-AP (same clone as 11289-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis of human kidney using 11289-1-AP (same clone as 11289-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis of rat heart using 11289-1-AP (same clone as 11289-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using PARP3 Polyclonal antibody (11289-1-AP)

    WB analysis of human heart using 11289-1-AP (same clone as 11289-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human pancreas cancer using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11289-1-AP (PARP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human breast cancer using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human renal cell carcinoma using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP3 Polyclonal antibody (11289-1-AP)

    IHC staining of human breast cancer using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PARP3 Polyclonal antibody (11289-1-AP)

    IF Staining of HeLa using 11289-1-AP (same clone as 11289-1-PBS)

    Immunofluorescent analysis of Hela cells, using PARP3 antibody 11289-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PARP3 Polyclonal antibody, PBS Only

PARP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11289-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hPARP 3, EC:2.4.2.-, DNA ADP-ribosyltransferase PARP3, ARTD3, ADPRT3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11289-1-PBS targets PARP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1844

Product name: Recombinant human PARP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 234-533 aa of BC014260

Sequence: EALEEALKGPTDGGQSLEELSSHFYTVIPHNFGHSQPPPINSPELLQAKKDMLLVLADIELAQALQAVSEQEKTVEEVPHPLDRDYQLLKCQLQLLDSGAPEYKVIQTYLEQTGSNHRCPTLQHIWKVNQEGEEDRFQAHSKLGNRKLLWHGTNMAVVAAILTSGLRIMPHSGGRVGKGIYFASENSKSAGYVIGMKCGAHHVGYMFLGEVALGREHHINTDNPSLKSPPPGFDSVIARGHTEPDPTQDTELELDGQQVVVPQGQPVPCPEFSSSTFSQSEYLIYQESQCRLRYLLEVHL

相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 3
Calculated molecular weight 60 kDa
Observed molecular weight 60-62 kDa
GenBank accession numberBC014260
Gene Symbol PARP3
Gene ID (NCBI) 10039
RRIDAB_2283392
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6F1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PARP3 (Poly(ADP-ribose) polymerase 3), also known as ARTD3, is the third member of the PARP family that catalyze a post-translational modification of proteins to promote, control or adjust numerous cellular events including genome integrity, transcription, differentiation, cell metabolism or cell death (PMID: 31095444). PARP3 is a 60-kDa protein containing an N-terminal WGR (tryptophan-, glycine-, and arginine-rich) domain and a C-terminal catalytic domain. PARP3 has been described to interact with partners belonging to the NHEJ pathway including DNA-PKcs, DNA ligase IV, Ku70 and Ku80 and to accelerate XRCC4/DNA ligase IV-mediated ligation of chromosomal DSB in concert with APLF (PMID: 24598253).

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