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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP2 Polyclonal antibody (55149-1-AP)

    WB analysis using 55149-1-AP (same clone as 55149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55149-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PARP2 Polyclonal antibody (55149-1-AP)

    IHC staining of human kidney using 55149-1-AP (same clone as 55149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55149-1-AP (PARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PARP2 Polyclonal antibody (55149-1-AP)

    IHC staining of mouse testis using 55149-1-AP (same clone as 55149-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55149-1-AP (PARP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PARP2 Polyclonal antibody (55149-1-AP)

    IF Staining of U2OS using 55149-1-AP (same clone as 55149-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55149-1-AP (PARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55149-1-PBS in a different storage buffer formulation.

PARP2 Polyclonal antibody, PBS Only

PARP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55149-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55149-1-PBS targets PARP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase 2
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_005484
Gene Symbol PARP2
Gene ID (NCBI) 10038
RRIDAB_10858796
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGN5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

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