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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of Jurkat using 26072-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of HL-60 using 26072-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of MCF-7 using 26072-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PARP10 Polyclonal antibody (26072-1-AP)

    IP experiment of Jurkat using 26072-1-AP

    IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PARP10 Polyclonal antibody (26072-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PARP10 Polyclonal antibody (26072-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PARP10 Polyclonal antibody (26072-1-AP)

    IF Staining of MCF-7 using 26072-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PARP10 antibody (26072-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PARP10 Polyclonal antibody (26072-1-AP)

    IF Staining of HepG2 using 26072-1-AP

    Immunofluorescent analysis of HepG2 cells using 26072-1-AP (PARP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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PARP10 Polyclonal antibody

PARP10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26072-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 10, ARTD10, EC:2.4.2.-, PARP 10, PARP-10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26072-1-AP targets PARP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP10 fusion protein Ag23457 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 10
Observed molecular weight 130-140 kDa
GenBank accession numberBC136542
Gene Symbol PARP10
Gene ID (NCBI) 84875
RRIDAB_2880362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP10 (Poly(ADP-ribose) polymerase family member 10) is a mono-ADP-ribosyltransferase that belongs to the PARP family. It regulates a variety of cellular functions, including gene transcription, DNA repair, and cell cycle control, by catalyzing ADP-ribosylation reactions. PARP10 is distributed in both the nucleus and cytoplasm, participating in the cellular response to DNA damage. In particular, its expression level significantly increases in response to UV-induced DNA damage. Additionally, PARP10 plays a crucial role in the antiviral response, being highly upregulated by interferon (IFN) to inhibit the replication of certain viruses and block protein translation during viral replication. PARP10 is also involved in immune regulation, interacting with NEMO to block the NF-κB signaling pathway and the production of pro-inflammatory cytokines.

Protocols

Product Specific Protocols
WB protocol for PARP10 antibody 26072-1-APDownload protocol
IHC protocol for PARP10 antibody 26072-1-APDownload protocol
IF protocol for PARP10 antibody 26072-1-APDownload protocol
IP protocol for PARP10 antibody 26072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols