Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
27808-1-AP targets PARG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27137 Product name: Recombinant human PARG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 183-441 aa of BC052966 Sequence: SNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPK 相同性解析による交差性が予測される生物種 |
| Full Name | poly (ADP-ribose) glycohydrolase |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 100-111 kDa |
| GenBank accession number | BC052966 |
| Gene Symbol | PARG |
| Gene ID (NCBI) | 8505 |
| RRID | AB_2880979 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86W56 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme that reverses the PARylation process by breaking down long chains of PAR polymers on proteins, thus having an opposite function to PARP. It hydrolyzes PAR into ADP-ribose units, terminating signals initiated by PARP enzymes. PARG's function is crucial for maintaining PAR balance within cells, which is essential for DNA repair, transcriptional regulation, and cell death. PARG possesses multiple isoforms with distinct subcellular localizations, and its activity is essential for cellular survival. (PMID: 38580335, PMID: 39546575)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PARG antibody 27808-1-AP | Download protocol |
| IHC protocol for PARG antibody 27808-1-AP | Download protocol |
| IP protocol for PARG antibody 27808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma. | ||
Int J Mol Sci Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum | ||
Onco Targets Ther Synergistic Effect of Ubiquitin-Specific Protease 14 and Poly(ADP-Ribose) Glycohydrolase Co-Inhibition in BRCA1-Mutant, Poly(ADP-Ribose) Polymerase Inhibitor-Resistant Triple-Negative Breast Cancer Cells | ||
Oncogene DNMT1 is required for efficient DSB repair and maintenance of replication fork stability, and its loss reverses resistance to PARP inhibitors in cancer cells | ||
Nat Chem Biol Stabilization of AFF1 by PARylation ensures transcriptional restart after DNA damage | ||
Viruses Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus |