Validation Data Gallery
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Tested Applications
| Positive WB detected in | MDCK cells, NIH/3T3 cells, RAW 264.7 cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
84593-1-RR targets PAR3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | coagulation factor II (thrombin) receptor-like 2 |
| Calculated molecular weight | 374 aa, 43 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC093648 |
| Gene Symbol | PAR3 |
| Gene ID (NCBI) | 2151 |
| RRID | AB_3672089 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O00254 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAR3 is a crucial protein involved in the establishment and maintenance of cell polarity across various species. It plays a significant role in organizing cellular structures and functions, particularly in epithelial and neuronal cells. PAR3 has been implicated in various aspects of cancer biology. It can act as a tumor suppressor in epithelial cancers, and its expression or mutations have been associated with the progression and behavior of glioblastoma, pancreatic cancer, and other malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAR3 antibody 84593-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |