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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAR1/Thrombin Receptor Polyclonal antibody (26366-1-AP)

    WB analysis using 26366-1-AP (same clone as 26366-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26366-1-AP (PAR1/Thrombin Receptor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26366-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PAR1/Thrombin Receptor Polyclonal antibody (26366-1-AP)

    WB analysis of K-562 using 26366-1-AP (same clone as 26366-1-PBS)

    WB result of F2R antibody (26366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAR1/Thrombin Receptor transfected K-562 cells. This data was developed using the same antibody clone with 26366-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PAR1/Thrombin Receptor Polyclonal antibody (26366-1-AP)

    WB analysis of mouse liver using 26366-1-AP (same clone as 26366-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26366-1-AP (F2R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26366-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PAR1/Thrombin Receptor Polyclonal antibody (26366-1-AP)

    IHC staining of human liver using 26366-1-AP (same clone as 26366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26366-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PAR1/Thrombin Receptor Polyclonal antibody (26366-1-AP)

    IHC staining of human liver using 26366-1-AP (same clone as 26366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26366-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PAR1/Thrombin Receptor Polyclonal antibody, PBS Only

PAR1/Thrombin Receptor Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26366-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PAR1, Thrombin receptor, CF2R, F2R, HTR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26366-1-PBS targets PAR1/Thrombin Receptor in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24378

Product name: Recombinant human F2R protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-102 aa of BC051909

Sequence: LLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLT

相同性解析による交差性が予測される生物種
Full Name coagulation factor II (thrombin) receptor
Calculated molecular weight 47 kDa
Observed molecular weight70 kDa, 47 kDa
GenBank accession numberBC051909
Gene Symbol F2R
Gene ID (NCBI) 2149
RRIDAB_2880492
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP25116
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

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