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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAPSS1 Polyclonal antibody

PAPSS1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14708-1-AP

Synonyms

APS kinase, ATP sulfurylase, ATPSK1, PAPS synthase 1, PAPSS, PAPSS 1, PAPSS1, SAT Adenylyl sulfate kinase, SK 1, SK1, Sulfate adenylate transferase, Sulfurylase kinase 1

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 14708-1-AP

    WB analysis of HepG2 using 14708-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 14708-1-AP

    WB analysis of Jurkat using 14708-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14708-1-AP (PAPSS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14708-1-AP

    IP experiment of HepG2 using 14708-1-AP

    IP result of anti-PAPSS1 (IP:14708-1-AP, 4ug; Detection:14708-1-AP 1:1000) with HepG2 cells lysate 1000ug.

  • IHC staining of human ovary tumor using 14708-1-AP

    IHC staining of human ovary tumor using 14708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14708-1-AP (PAPSS1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary tumor using 14708-1-AP

    IHC staining of human ovary tumor using 14708-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14708-1-AP (PAPSS1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 14708-1-AP

    IF Staining of HepG2 using 14708-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14708-1-AP (PAPSS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14708-1-AP targets PAPSS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAPSS1 fusion protein Ag6401 相同性解析による交差性が予測される生物種
Full Name 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Calculated molecular weight 71 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC050627
Gene symbol PAPSS1
Gene ID (NCBI) 9061
RRIDAB_2159487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PAPSS1 antibody 14708-1-APDownload protocol
IHC protocol for PAPSS1 antibody 14708-1-APDownload protocol
IF protocol for PAPSS1 antibody 14708-1-APDownload protocol
IP protocol for PAPSS1 antibody 14708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,CoIP

Cancer Res

CIB2 negatively regulates oncogenic signaling in ovarian cancer via sphingosine kinase 1.

Authors - Wenying Zhu
View Article
hamsterWB

Biotechnol J

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.

Authors - Ren Liu
View Article
humanIHC

Histol Histopathol

Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma

Authors - Xuyou Zhu
View Article