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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAPPA Polyclonal antibody

PAPPA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18095-1-AP

Synonyms

ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPPA1, Pappalysin 1

Validation Data Gallery

Applications:
  • WB analysis using 18095-1-AP

    WB analysis using 18095-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 18095-1-AP (PAPPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18095-1-AP

    IHC staining of human placenta using 18095-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included
  • IHC staining of human placenta using 18095-1-AP

    IHC staining of human placenta using 18095-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAPPA Ready-To-Use IHC Kit. PAPPA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18095-1-AP targets PAPPA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAPPA fusion protein Ag11863 相同性解析による交差性が予測される生物種
Full Name pregnancy-associated plasma protein A, pappalysin 1
Calculated molecular weight 1627 aa, 181 kDa
Observed molecular weight180-220 kDa
GenBank accession numberBC078657
Gene symbol PAPPA
Gene ID (NCBI) 5069
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

Protocols

Product Specific Protocols
WB protocol for PAPPA antibody 18095-1-APDownload protocol
IHC protocol for PAPPA antibody 18095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols