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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PAPOLG Polyclonal antibody (24284-1-AP)

    WB analysis of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PAPOLG Polyclonal antibody (24284-1-AP)

    WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PAPOLG Polyclonal antibody (24284-1-AP)

    WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PAPOLG Polyclonal antibody (24284-1-AP)

    IP experiment of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

    IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PAPOLG Polyclonal antibody (24284-1-AP)

    IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PAPOLG Polyclonal antibody (24284-1-AP)

    IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PAPOLG Polyclonal antibody (24284-1-AP)

    IF Staining of HepG2 using 24284-1-AP (same clone as 24284-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

PAPOLG Polyclonal antibody, PBS Only

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24284-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24284-1-PBS targets PAPOLG in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16208

Product name: Recombinant human PAPOLG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 469-736 aa of BC111701

Sequence: QANNINMLKEGMKIEATHVKKKQLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETERNSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPHLNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDTSRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR

相同性解析による交差性が予測される生物種
Full Name poly(A) polymerase gamma
Calculated molecular weight 736 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC111701
Gene Symbol PAPOLG
Gene ID (NCBI) 64895
RRIDAB_2879471
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWT3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

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