Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | rat stomach tissue, human placenta tissue, mouse kidney tissue, mouse lung tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
55197-1-AP targets PAPD5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | PAP associated domain containing 5 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 63-80 kDa |
| GenBank accession number | NM_001040285 |
| Gene Symbol | PAPD5 |
| Gene ID (NCBI) | 64282 |
| RRID | AB_2881287 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NDF8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAPD5 antibody 55197-1-AP | Download protocol |
| IP protocol for PAPD5 antibody 55197-1-AP | Download protocol |
| WB protocol for PAPD5 antibody 55197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The MTR4/hnRNPK complex surveils aberrant polyadenylated RNAs with multiple exons | ||
Nat Commun Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5 | ||
Nucleic Acids Res mRNAs are sorted for export or degradation before passing through nuclear speckles.
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