Validation Data Gallery
Tested Applications
| Positive IHC detected in | Human brain cancer (Astrocitoma) Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98696-1-RR targets PANX2 in IHC, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | pannexin 2 |
| Calculated molecular weight | 677 aa, 74 kDa |
| GenBank accession number | BC101023 |
| Gene Symbol | PANX2 |
| Gene ID (NCBI) | 56666 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96RD6 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Pannexins are glycoproteins that form functional single-membrane channels, which mediate ATP release in response to a variety of physiological and pathological stimuli (PMID: 33913372). The pannexin family consists of three members, namely PANX1, PANX2, and PANX3 (PMID: 22305965). PANX2 was initially predicted to be expressed selectively in the central nervous system, but was later shown to have broad expression across multiple tissues, including skin, skeletal muscle, eye, and colon (PMID: 15028292; 25505382; 25773474). PANX2 is involved in pathophysiological processes of neurological and cancer diseases (PMID: 19749789; 33913372; 39389335).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PANX2 antibody 98696-1-RR | Download protocol |
| IHC protocol for PANX2 antibody 98696-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |