Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32953-1-AP targets PANK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38232 Product name: Recombinant human PANK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of NM_018216.2 Sequence: MAECGASGSGSSGDSLDKSITLPPDEIFRNLENAKRFAIDIGGSLTKLAYYSTVQHKVAKVRSFDHSGKDTEREHEPPYEISVQEEITARLHFIKFENTYIEACLDFIKDHLVNTETKVIQATGGGAYKFKDLIEEKLRLKVDKEDVMTCLIKGCNFVLKNIPHEAFVYQKDSDPEFR 相同性解析による交差性が予測される生物種 |
| Full Name | pantothenate kinase 4 |
| Calculated molecular weight | 86 kDa，773aa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | NM_018216.2 |
| Gene Symbol | PANK4 |
| Gene ID (NCBI) | 55229 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9NVE7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PANK4 (Pantothenate Kinase 4) is a member of the pantothenate kinase family, which is involved in the biosynthesis of coenzyme A (CoA). However, unlike other members of the family, PANK4 lacks the residues required for kinase activity and instead contains a phosphatase domain (DUF89) that can dephosphorylate 4'-phosphopantetheine, an intermediate in CoA biosynthesis (PMID: 30927326). This suggests that PANK4 may act as a pseudoenzyme, regulating cellular CoA levels by antagonizing CoA production (PMID: 39746949).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PANK4 antibody 32953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |