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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PANK2 Polyclonal antibody (11001-1-AP)

    WB analysis using 11001-1-AP (same clone as 11001-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using PANK2 Polyclonal antibody (11001-1-AP)

    WB analysis of human brain using 11001-1-AP (same clone as 11001-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PANK2 Polyclonal antibody (11001-1-AP)

    IP experiment of mouse brain using 11001-1-AP (same clone as 11001-1-PBS)

    IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PANK2 Polyclonal antibody (11001-1-AP)

    IHC staining of human lung cancer using 11001-1-AP (same clone as 11001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PANK2 Polyclonal antibody (11001-1-AP)

    IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PANK2 Polyclonal antibody (11001-1-AP)

    IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PANK2 Polyclonal antibody (11001-1-AP)

    IF Staining of HepG2 using 11001-1-AP (same clone as 11001-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

PANK2 Polyclonal antibody, PBS Only

PANK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11001-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Pantothenate kinase 2, mitochondrial intermediate form, mPanK2, iPanK2, hPanK2, EC:2.7.1.33

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11001-1-PBS targets PANK2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1413

Product name: Recombinant human PANK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 328-570 aa of BC009421

Sequence: IGDLQLCKLDELDCLIKGILYIDSVGFNGRSQCYYFENPADSEKCQKLPFDLKNPYPLLLVNIGSGVSILAVYSKDNYKRVTGTSLGGGTFFGLCCLLTGCTTFEEALEMASRGDSTKVDKLVRDIYGGDYERFGLPGWAVASSFGNMMSKEKREAVSKEDLARATLITITNNIGSIARMCALNENINQVVFVGNFLRINTIAMRLLAYALDYWSKGQLKALFSEHEGYFGAVGALLELLKIP

相同性解析による交差性が予測される生物種
Full Name pantothenate kinase 2
Calculated molecular weight 63 kDa
Observed molecular weight63-70 kDa
GenBank accession numberBC009421
Gene Symbol PANK2
Gene ID (NCBI) 80025
RRIDAB_2159041
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZ23
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.

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