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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    WB analysis of HepG2 using 12466-1-AP (same clone as 12466-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12466-1-AP (PANK1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human hepatocirrhosis using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human hepatocirrhosis using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human liver cancer using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human breast cancer using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PANK1/2/3 Polyclonal antibody (12466-1-AP)

    IHC staining of human breast cancer using 12466-1-AP (same clone as 12466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12466-1-PBS in a different storage buffer formulation.

PANK1/2/3 Polyclonal antibody, PBS Only

PANK1/2/3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12466-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12466-1-PBS targets PANK1/2/3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3140

Product name: Recombinant human PANK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-370 aa of BC013705

Sequence: TGIRDVHLELKDLTLFGRRGNLHFIRFPTQDLPTFIQMGRDKNFSTLQTVLCATGGGAYKFEKDFRTIGNLHLHKLDELDCLVKGLLYIDSVSFNGQAECYYFANASEPERCQKMPFNLDDPYPLLVVNIGSGVSILAVHSKDNYKRVTGTSLGGGTFLGLCSLLTGCESFEEALEMASKGDSTQADKLVRDIYGGDYERFGLPGWAVASSFGNMIYKEKRESVSKEDLARATLVTITNNIGSVARMCAVNEKINRVVFVGNFLRVNTLSMKLLAYALDYWSKGQLKALFLEHEGYFGAVGALLGLPNFS

相同性解析による交差性が予測される生物種
Full Name pantothenate kinase 3
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC013705
Gene Symbol PANK3
Gene ID (NCBI) 79646
RRIDAB_10644117
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H999
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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