Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
87921-1-PBS targets PAM in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5194 Product name: Recombinant human PAM protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 21-494 aa of NM_001177306.2 Sequence: FRSPLSVFKRFKETTRPFSNECLGTTRPVVPIDSSDFALDIRMPGVTPKQSDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESESDLVAEIANVVQKKDLGRSDAREGAEHERGNAILVRDRIHKFHRLVSTLRPPESRVFSLQQPPPGEGTWEPEHTG 相同性解析による交差性が予測される生物種 |
| Full Name | peptidylglycine alpha-amidating monooxygenase |
| Calculated molecular weight | 108 kDa |
| Observed molecular weight | 75 kDa and 105-120 kDa |
| GenBank accession number | NM_001177306.2 |
| Gene Symbol | PAM |
| Gene ID (NCBI) | 5066 |
| RRID | AB_3745828 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19021-1 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Peptidyl-glycine alpha-amidating monooxygenase (PAM) is a bifunctional type I membrane protein essential for neuropeptide maturation. It catalyzes the C-terminal amidation of peptides via two sequential enzymatic domains: peptidylglycine α-hydroxylating monooxygenase (PHM, ~44-46 kDa) and peptidyl-α-hydroxyglycine α-amidating lyase (PAL, ~50 kDa), which together convert glycine-extended precursors into bioactive amidated peptides. PAM undergoes alternative splicing, generating integral membrane isoforms such as PAM-1 (~120 kDa) and PAM-2 (~105 kDa). Endoproteolytic cleavage produces soluble monofunctional PHM and PAL, as well as a bifunctional ~75 kDa fragment. The cleaved cytosolic domain (sfCD, ~16 kDa) can translocate to the nucleus, influencing gene expression. PAM also supports angiogenesis and tumor progression, notably in glioblastoma, highlighting its therapeutic potential (PMID: 8436961; PMID: 32054826).