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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PALMD Polyclonal antibody (16531-1-AP)

    WB analysis of HeLa using 16531-1-AP (same clone as 16531-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PALMD Polyclonal antibody (16531-1-AP)

    WB analysis of HeLa using 16531-1-AP (same clone as 16531-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using PALMD Polyclonal antibody (16531-1-AP)

    WB analysis of mouse lung using 16531-1-AP (same clone as 16531-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using PALMD Polyclonal antibody (16531-1-AP)

    IP experiment of mouse heart using 16531-1-AP (same clone as 16531-1-PBS)

    IP result of anti-PALMD (IP:16531-1-AP, 5ug; Detection:16531-1-AP 1:500) with mouse heart tissue lysate 6000ug. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment using PALMD Polyclonal antibody (16531-1-AP)

    IP experiment using 16531-1-AP (same clone as 16531-1-PBS)

    IP result of PALMD antibody (16531-1-AP for Ip and Detection) with mouse heart tissue lysate. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PALMD Polyclonal antibody (16531-1-AP)

    IHC staining of human liver using 16531-1-AP (same clone as 16531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PALMD Polyclonal antibody (16531-1-AP)

    IHC staining of human liver using 16531-1-AP (same clone as 16531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PALMD Polyclonal antibody, PBS Only

PALMD Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16531-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16531-1-PBS targets PALMD in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PALMD fusion protein Ag9796 相同性解析による交差性が予測される生物種
Full Name palmdelphin
Calculated molecular weight 551 aa, 63 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC012570
Gene Symbol PALMD
Gene ID (NCBI) 54873
RRIDAB_2236613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP74
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.