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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Palladin Monoclonal antibody (66601-1-Ig)

    WB analysis of human heart using 66601-1-Ig (same clone as 66601-1-PBS)

    Human heart tissue, pig heart tissue and rat heart tissue were subjected to SDS PAGE followed by western blot with 66601-1-Ig (Palladin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66601-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Palladin Monoclonal antibody (66601-1-Ig)

    IHC staining of human colon cancer using 66601-1-Ig (same clone as 66601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66601-1-Ig (Palladin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66601-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Palladin Monoclonal antibody (66601-1-Ig)

    IHC staining of human colon cancer using 66601-1-Ig (same clone as 66601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66601-1-Ig (Palladin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66601-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Palladin Monoclonal antibody (66601-1-Ig)

    IF Staining of mouse testis using 66601-1-Ig (same clone as 66601-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PALLD,palladin antibody (66601-1-Ig, Clone: 2A1F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66601-1-PBS in a different storage buffer formulation.

Palladin Monoclonal antibody, PBS Only

Palladin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66601-1-PBS
Clone No.2A1F6

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CGI 151, KIAA0992, palladin, PALLD, PALLD,palladin, PNCA1, Sarcoma antigen NY SAR 77, SIH002

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66601-1-PBS targets Palladin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1288

Product name: Recombinant human PALLD,palladin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-510 aa of BC013867

Sequence: APFFEMKLKHYKIFEGMPVTFTCRVAGNPKPKIYWFKDGKQISPKSDHYTIQRDLDGTCSLHTTASTLDDDGNYTIMAANPQGRISCTGRLMVQAVNQRGRSPRSPSGHPHVRRPRSRSRDSGDENEPIQERFFRPHFLQAPGDLTVQEGKLCRMDCKVSGLPTPDLSWQLDGKPVRPDSAHKMLVRENGVHSLIIEPVTSRDAGIYTCIATNRAGQNSFSLELVVAAKEAHKPPVFIEKLQNTGVADGYPVRLECRVLGVPPPQIFWKKENESLTHSTDRVSMHQDNHGYICLLIQGATKEDAGWYTVSAKNEAGIVSCTARLDVYTQWHQQSQSTKPKKVRPSASRYAALSDQGLDIKAAFQPEANPSHLTLNTALVESEDL

相同性解析による交差性が予測される生物種
Full Name palladin, cytoskeletal associated protein
Calculated molecular weight 1383 aa, 151 kDa
Observed molecular weight 151 kDa
GenBank accession numberBC013867
Gene Symbol palladin
Gene ID (NCBI) 23022
RRIDAB_2881961
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WX93
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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