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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAK7 Recombinant antibody

PAK7 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230201A10

Cat no : 82935-1-RR

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

Validation Data Gallery

Applications:
  • WB analysis using 82935-1-RR

    WB analysis using 82935-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 82935-1-RR

    IF Staining of U-251 using 82935-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (82935-1-RR, Clone: 230201A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

82935-1-RR targets PAK7 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAK7 fusion protein Ag29190 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight70 kDa
GenBank accession numberBC024179
Gene symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

Protocols

Product Specific Protocols
WB protocol for PAK7 antibody 82935-1-RRDownload protocol
IF protocol for PAK7 antibody 82935-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols