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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis using 51137-1-AP (same clone as 51137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis of HEK-293 using 51137-1-AP (same clone as 51137-1-PBS)

    WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis of K-562 using 51137-1-AP (same clone as 51137-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PAK1 Polyclonal antibody (51137-1-AP)

    WB analysis of A431 using 51137-1-AP (same clone as 51137-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PAK1 Polyclonal antibody (51137-1-AP)

    IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PAK1 Polyclonal antibody (51137-1-AP)

    IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PAK1 Polyclonal antibody (51137-1-AP)

    IF Staining of HeLa using 51137-1-AP (same clone as 51137-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PAK1 Polyclonal antibody, PBS Only

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 51137-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51137-1-PBS targets PAK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 1
Calculated molecular weight 553 aa, 62 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC109299
Gene Symbol PAK1
Gene ID (NCBI) 5058
RRIDAB_2283523
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13153
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

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