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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    WB analysis of human brain using 20564-1-AP (same clone as 20564-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20564-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    WB analysis using 20564-1-AP (same clone as 20564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    IHC staining of human spleen using 20564-1-AP (same clone as 20564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    IHC staining of human spleen using 20564-1-AP (same clone as 20564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    IF Staining of SH-SY5Y using 20564-1-AP (same clone as 20564-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20564-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PAFAH1B3 Polyclonal antibody, PBS Only

PAFAH1B3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20564-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:3.1.1.47, PAF-AH 29 kDa subunit, PAF-AH subunit gamma, PAFAHG, Platelet-activating factor acetylhydrolase IB subunit alpha1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20564-1-PBS targets PAFAH1B3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa
Calculated molecular weight 26 kDa
Observed molecular weight 26-29 kDa
GenBank accession numberNM_002573
Gene Symbol PAFAH1B3
Gene ID (NCBI) 5050
RRIDAB_10693534
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15102
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PAFAH1B3, also named as PAFAHG, belongs to the 'GDSL' lipolytic enzyme family and Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. It inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. PAFAH1B3 plays an important role during the development of brain. The antibody is specific to PAFAH1B3.

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