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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated PADI2 Monoclonal antibody (CL594-66386)

    IF Staining of human breast cancer using CL594-66386

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66386 (PADI2 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated PADI2 Monoclonal antibody (CL594-66386)

    IF Staining of HeLa using CL594-66386

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 PADI2 antibody (CL594-66386, Clone: 2D8C12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated PADI2 Monoclonal antibody (CL594-66386)

    FC experiment of HeLa using CL594-66386

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PADI2 (CL594-66386, Clone:2D8C12) (red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PADI2 Monoclonal antibody

PADI2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-66386
Clone No.2D8C12

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman breast cancer tissue, HeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66386 targets PADI2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17612

Product name: Recombinant human PADI2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 118-438 aa of BC009701

Sequence: ISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPL

相同性解析による交差性が予測される生物種
Full Name peptidyl arginine deiminase, type II
Calculated molecular weight 665 aa, 75 kDa
GenBank accession numberBC009701
Gene Symbol PADI2
Gene ID (NCBI) 11240
RRIDAB_2883573
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2J8
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa. This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 PADI2 antibody CL594-66386Download protocol
FC protocol for CL594 PADI2 antibody CL594-66386Download protocol
Standard Protocols
Click here to view our Standard Protocols