Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 12110-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12110-1-AP
WB result of PADI2 antibody (12110-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.
WB analysis of MCF-7 using 12110-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12110-1-AP
IP result of anti-PADI2 (IP:12110-1-AP, 4ug; Detection:12110-1-AP 1:2000) with mouse brain tissue lysate 1720 ug.
IHC staining of human breast cancer using 12110-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12110-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12110-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12110-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PADI2 antibody (12110-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human breast cancer using 12110-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 12110-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PADI2 antibody (12110-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12110-1-AP

Publications(80)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA, Blocking assay

Synonyms

EC:3.5.3.15, KIAA0994, PAD H19, PAD2, PAD-H19

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (80)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, MCF-7 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue, human breast cancer tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 17 publications below
WB	See 54 publications below
IHC	See 16 publications below
IF	See 25 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

12110-1-AP targets PADI2 in WB, IHC, IF-P, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2755 Product name: Recombinant human PADI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-438 aa of BC009701 Sequence: ISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPL 相同性解析による交差性が予測される生物種
Full Name	peptidyl arginine deiminase, type II
Calculated molecular weight	665 aa, 75 kDa
Observed molecular weight	70-75 kDa, 50 kDa
GenBank accession number	BC009701
Gene Symbol	PADI2
Gene ID (NCBI)	11240
RRID	AB_2159475
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2J8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Protocols

Product Specific Protocols
IHC protocol for PADI2 antibody 12110-1-AP	Download protocol
IP protocol for PADI2 antibody 12110-1-AP	Download protocol
WB protocol for PADI2 antibody 12110-1-AP	Download protocol
IF protocol for PADI2 antibody 12110-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human, mouse	WB,IF	Immunity Astrocytic calcium-dependent enzyme PAD2 governs microglia activity to exacerbate amyloid pathology via citrullinated vimentin Authors - Jingyan Zhang View Article
mouse	WB,IHC,IF	Cancer Res PAD2 Overexpression in Transgenic Mice Promotes Spontaneous Skin Neoplasia. Authors - John L McElwee KD Validated View Article
human	WB,IHC,IF,COIP	Cancer Res PADI2-mediated citrullination promotes prostate cancer progression. Authors - Lin Wang KD Validated View Article
mouse	IF	Nat Neurosci Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice Authors - Timothy M O'Shea View Article
mouse	WB	Nat Commun A citrullinated histone H3 monoclonal antibody for immune modulation in sepsis. Authors - Wenlu Ouyang View Article
rat,human	IHC,IF	Nat Commun Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis Authors - Yibing Han View Article

Validation Data Gallery

WB analysis using 12110-1-AP

WB analysis of MCF-7 using 12110-1-AP

WB analysis of MCF-7 using 12110-1-AP

IP experiment of mouse brain using 12110-1-AP

IHC staining of human breast cancer using 12110-1-AP

IHC staining of human prostate cancer using 12110-1-AP

IHC staining of human breast cancer using 12110-1-AP

IF Staining of mouse brain using 12110-1-AP

IF Staining of human breast cancer using 12110-1-AP

IF Staining of HeLa using 12110-1-AP

PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Astrocytic calcium-dependent enzyme PAD2 governs microglia activity to exacerbate amyloid pathology via citrullinated vimentin

PAD2 Overexpression in Transgenic Mice Promotes Spontaneous Skin Neoplasia.

PADI2-mediated citrullination promotes prostate cancer progression.

Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice

A citrullinated histone H3 monoclonal antibody for immune modulation in sepsis.

Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis

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