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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PACT Polyclonal antibody (28636-1-AP)

    WB analysis using 28636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PACT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PACT Polyclonal antibody (28636-1-AP)

    WB analysis of HeLa using 28636-1-AP

    WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected HeLa cells.

  • Western Blot (WB) analysis of MCF-7 cells using PACT Polyclonal antibody (28636-1-AP)

    WB analysis of MCF-7 using 28636-1-AP

    WB result of PACT antibody (28636-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACT transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using PACT Polyclonal antibody (28636-1-AP)

    WB analysis using 28636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28636-1-AP (PRKRA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

PACT Polyclonal antibody

PACT Polyclonal Antibody for WB, ELISA
Cat No. 28636-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, MCF-7 cells, rat restis tissue, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28636-1-AP targets PACT in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PACT fusion protein Ag29784 相同性解析による交差性が予測される生物種
Full Name protein kinase, interferon-inducible double stranded RNA dependent activator
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC009470
Gene Symbol PRKRA
Gene ID (NCBI) 8575
RRIDAB_2918186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

Protocols

Product Specific Protocols
WB protocol for PACT antibody 28636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols