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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis of HeLa using 10639-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PACSIN3 Polyclonal antibody (10639-1-AP)

    WB analysis using 10639-1-AP

    Mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    IHC staining of human breast cancer using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    IHC staining of human heart using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PACSIN3 Polyclonal antibody (10639-1-AP)

    IHC staining of human heart using 10639-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PACSIN3 Polyclonal antibody (10639-1-AP)

    IF Staining of HeLa using 10639-1-AP

    Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PACSIN3 Polyclonal antibody

PACSIN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10639-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Protein kinase C and casein kinase substrate in neurons protein 3, SH3 domain-containing protein 6511

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10639-1-AP targets PACSIN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1006

Product name: Recombinant human PACSIN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC007914

Sequence: MAPEEDAGGEALGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGTVEKGPQYGTLEKAWHAFFTAAERLSALHLEVREKLQGQDSERVRAWQRGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHAARKDEKTAQTRESHAKADSAVSQEQLRKLQERVERCAKEAEKTKAQYEQTLAELHRYTPRYMEDMEQAFETCQAAERQRLLFFKDMLLTLHQHLDLSSSEKFHELHRDLHQGIEAASDEEDLRWWRSTHGPGMAMNWPQFE

相同性解析による交差性が予測される生物種
Full Name protein kinase C and casein kinase substrate in neurons 3
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC007914
Gene Symbol PACSIN3
Gene ID (NCBI) 29763
RRIDAB_2159453
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PACSIN3 antibody 10639-1-APDownload protocol
IHC protocol for PACSIN3 antibody 10639-1-APDownload protocol
WB protocol for PACSIN3 antibody 10639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

Biallelic truncating variants in PACSIN3 cause childhood-onset myopathy with hyperCKaemia

Authors - Felix Distelmaier
View Article
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